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AssigneeNanodigmbio (Nanjing) Biotechnology Co., Ltd.
InventorsYugang HU, Yan QU, Biao WANG, Tong LI, Qiang WU
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Provided in the present application is a method for constructing a DNA library based on an MGI platform and applications thereof. The method includes: ligating a bubble adapter to a target sample to obtain a ligation product; and performing library construction on the ligation product utilizing an amplification primer pair, obtaining an MGI platform based amplified library with dual indexes, where the amplification primer pair has the dual indexes and includes a 5′ end library index and a 3′ end library index; and the 5′ end library index is selected from indexes corresponding to any one of groups of 4-base-balanced phosphorylation ends in Table 1 or Table 2, and the 3′ end library index is selected from indexes corresponding to fixedly-matched non-phosphorylation ends corresponding to the 5′ end library index group in Table 1 or Table 2.
A method for constructing a DNA library based on an MGI platform, comprising:
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