Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology
InventorsFeng Zhang, Neville Espi Sanjana, Ophir Shalem
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Abstract
The present invention generally relates to compositions, methods applications and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
Claims16
1-30. (canceled)
A composition for functional genomic screening, comprising a population of CRISPR-edited eukaryotic cells each comprising a polynucleotide sequence encoding a Cas9 protein, and a mutation produced by a CRISPR-Cas9 complex formed by the Cas9 protein and a CRISPR-Cas9 system guide RNA, wherein each gene in human genome is mutated in the population of CRISPR-edited eukaryotic cells.
Dependencies are inferred from the claim text.