Subtilase variants and polynucloetides encoding same
AssigneeNovozymes A/S
InventorsFrank Winther Rasmussen, Peter Kamp Hansen, Lars Lehmann Hylling Christensen
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
Claims20
A subtilase variant having protease activity and having at least 25% improved stability compared to a subtilase with SEQ ID NO:
- 1, when measured as described in the accelerated storage stability assay in Example 3 herein, wherein the amino acid sequence of the subtilase variant is a polypeptide that has at least 60% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1 and wherein the subtilisin variant comprises substitutions in positions 160 and 209, wherein each position corresponds to the position of the polypeptide of SEQ ID NO:
- 2.
(canceled)
(canceled)
(canceled)
A method for producing a subtilase variant having protease activity and having at least 25% improved stability compared to a subtilase with SEQ ID NO:
- 1, comprising
- a. introducing into a parent subtilase one or more alterations from the group consisting of S9C, S9D, S9E, S9Q, N43A, N43C, N43L, N43R, N43W, I72A, I72V, S78D, V104F, A114V, G115T, G115W, H120I, H120T, H120V, P129D, V147W, V149C, V149N, A158E, G160D, G160P, S161C, S161E, S163A, S163D, Q182C, Q182E, N185C, N185E, S188C, S188D, S188E, Q19 IN, N204D, N204V, V205I, Q206C, Q206E, Q206I, Q206K, Q206T, Q206V, Q206W, Q206L Y209W, S212A, S212D, S212G, S212N, S216I, S216T, S216V, L217C, L217M, N218T, M222C, M222R, P225A, T255C, T255E, S256A, S256C, S256D, S256V, S256Y, S259D, T260E, T260P, N261C, N261E, N261L, N261M, N261V, N261Y, N261W, N261F, L262C, L262E and L262Q, wherein each position corresponds to the position of SEQ ID NO:
- 2 and
- b. recovering the variant.
Dependencies are inferred from the claim text.