Crispr-cas system using molecular chaperone to enhance trans-cleavage activity and application thereof
AssigneeInnovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences
InventorsLichun HE, Yihao CHEN, Xu ZHANG, Maili LIU
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Abstract
A CRISPR-Cas system using a molecular chaperone to enhance trans-cleavage activity and an application thereof are provided. The CRISPR-Cas system includes: 1 pM-1 mM Cas enzyme, 1 pM-1 mM crRNA, 1*reaction buffer, 0.1 pM-1 mM target substrate, 1 nM-1 mM ssDNA reporter gene, and 1 pM-1 mM molecular chaperone. The above design adds a molecular chaperone selected from ClpB, HSP70, Spy, or HSP20 to the CRISPR-Cas system, thereby increasing the trans-cleavage activity of the Cas enzyme to different extents and enhancing detection sensitivity.
Claims13
A CRISPR-Cas system using a molecular chaperone to enhance trans-cleavage activity, comprising:
- 1 pM-1 mM Cas enzyme, 1 pM-1 mM crRNA, 1*reaction buffer, 0.1 pM-1 mM target substrate, 1 nM-1 mM ssDNA reporter gene, and 1 pM-1 mM molecular chaperone.
Dependencies are inferred from the claim text.