Engineered nuclease with high salt tolerance
AssigneeWuxi Biologics Ireland Limited
InventorsShanhui LIAO, Cunjia QIU, Xiaoyue CHEN, Jiexing CAI, Weichang ZHOU
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Abstract
Provided is an engineered nuclease with high salt tolerance. The polypeptide comprises one or more mutations so that the polypeptide possesses more positive charged surface area in its three-dimensional structure. The polypeptide has at least 60% nuclease activity under a solution ionic strength more than 200 mM as compared to the nuclease having the sequence of SEQ ID NO: 1.
Claims20
A polypeptide derived from Serratia marcescens nuclease A, and having high salt tolerance, the polypeptide comprises one or more mutations so that the polypeptide possesses more positive charged surface area in its three-dimensional structure.
Dependencies are inferred from the claim text.