Methods for cell surface remodeling
AssigneesNantes Universite, Institut National de la Sante et de la Recherche Medicale, Centre National De La Recherche Scientifique
InventorsSÉBASTIEN GOUIN, DAVID DENIAUD, MATHIEU MEVEL, SÉBASTIEN DEPIENNE, DIMITRI ALVAREZ-DORTA, MOHAMMED BOUZELHA
Life of this patent
Estimating the term…
Abstract
The Invention relates to an in vitro method for chemically-modifying the surface of a cell, which comprises incubating said cell with N-substituted luminol moiety or a N-substituted phenylurazole in conditions conducive for reacting said chemical reagent with a cell surface component so as to form a covalent bond. The Invention also relates to a cell obtainable or obtained by such a process and its use in therapeutic, diagnostic or research field.
Claims16
1-19. (canceled)
An in vitro method for chemically-modifying the surface of a cellular entity, which comprises incubating said cellular entity with a chemical reagent bearing a N-substituted luminol moiety or a N-substituted phenyl-urazole in conditions conducive for reacting said chemical reagent with a surface component of said cellular entity so as to form a covalent bond and wherein the cellular entity is a cell or a extracellular vesicle.
A cellular entity having at least one chemically-modified tyrosine residue present in a cell surface component or a cell surface protein, which is of formula (C) or (CP):
- or formula (C-1) or (C-2) as follows:
- wherein:
- k is 1 or 2,
- RA is C1-C3 alkyl, a phenyl optionally substituted, a benzyl optionally substituted or -(Y)n-M,
- Ra1 is RA or H,
- each RB1 is independently a group of formula -(Y)n-M, a hydrogen or a substituent selected from the group consisting of a halogen, C1-C6 alkyl, C6-C14 aryl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 alkylamino, C2-C6 heterocycle, C1-C6 alkanoyl, C1-C6 carboxy esters, C1-C6 acylamino, —COOH, —CONH2, —NO2, —SO3H, —CN, —CF3, C1-C6 hydroxyalkyl, C1-C6 haloalkyl, C1-C6 thioalkyl, C1-C6 alkylthio, C2-C10 alkoxyalkyl, and C2-C6 alkoxycarbonyloxy,
- with proviso that when the chemically modified tyrosine is of formula (C), (C-1) or (C-2), at least one group among RA and RB1 groups is -(Y)n-M and when the chemical reagent is of formula (CP), at least one group among Ra1 and Ra1 groups is -(Y)n-M.n is 0 or 1,
- Y is a spacer, and
- M is a functional moiety.
Dependencies are inferred from the claim text.