Systems and methods of biomass monitoring for cell culture bioreactors
OwnerYe Fang, Kathleen Anne Krebs +1inventors
InventorsYe Fang, Kathleen Anne Krebs, Yujian Sun
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Abstract
A method of monitoring biomass during a cell culture of cells in a bioreactor is provided. The method includes culturing the cells in the bioreactor using a cell culture medium perfused through the bioreactor; measuring at least one of a cell nutrient and a cell byproduct in the cell culture medium; determining at least one of a consumption rate of the cell nutrient and an accumulation rate of the cell byproduct; and predicting a cell number within the bioreactor at a specified culture time based on at least one of the consumption rate and the accumulation rate.
Claims25
A method of monitoring biomass during a cell culture of cells in a bioreactor, the method comprising:
- culturing the cells in the bioreactor using a cell culture medium perfused through the bioreactor;
- measuring at least one of a cell nutrient and a cell byproduct in the cell culture medium;
- determining at least one of a consumption rate of the cell nutrient and an accumulation rate of the cell byproduct;
- predicting a cell number within the bioreactor at a specified culture time based on at least one of the consumption rate and the accumulation rate,
- wherein the measuring the at least one of the cell nutrient and the cell byproduct in the cell culture medium comprises taking multiple measurements of the cell nutrient or the cell byproduct, the multiple measurements being separated by a measurement interval that is less than a doubling time of the cells in the cell culture.
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Dependencies are inferred from the claim text.