Compositions and methods for enhancing donor oligonucleotide-based gene editing
AssigneeYale University
InventorsElias Quijano, Audrey Turchick, Peter Glazer
Life of this patent
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Abstract
Compositions for enhanced gene editing and methods of use thereof are. The composition contains a cell-penetrating antibody and a donor oligonucleotide containing a sequence that can correct a mutation in a cell's genome. Preferably, the composition does not contain a nuclease, PNA, or nanoparticle. The compositions are used to modify the genome of a cell by contacting the cell with an effective amount of the composition. Genomic modification occurs at a higher frequency both ex vivo and in vivo, when cells are contacted with the cell-penetrating antibody and donor oligonucleotide as compared to the absence of the cell-penetrating antibody.
Claims17
A composition comprising:
- (i) a donor oligonucleotide comprising a sequence that can correct at least one mutation in a cell's genome, and
- (ii) an antibody or antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof comprises:
- (a) a heavy chain variable region (VH) complementary determining region (CDR) 1 comprising the amino acid sequence of SEQ ID NO:
- 16,
- (b) a VH CDR2 comprising the amino acid sequence of SEQ ID NO:
- 17,
- (c) a VH CDR3 comprising the amino acid sequence of SEQ ID NO:
- 18,
- (d) a light chain variable region (VL) CDR1 comprising the amino acid sequence of SEQ ID NO:
- 24,
- (e) a VL CDR2 comprising the amino acid sequence of SEQ ID NO:
- 25,
- (f) a VL CDR3 comprising the amino acid sequence of SEQ ID NO:
- 26.
2-9. (canceled)
18-48. (canceled)
Dependencies are inferred from the claim text.