Antibody or peptide binding specifically to rnase a, immunoassay device and method for detecting rnase a, and method for collecting rnase a

An RNase detection immunoassay device, comprising:

• a substrate having a reaction space capable of accommodating a detection sample solution;
• a binding antibody located within the reaction space and fixed to the substrate;
• a switching peptide comprising a peptide compound reversibly bound to a Fab region of the binding antibody and a fluorescent label bound to the peptide compound;
• and
• a quenching material disposed adjacent to the fluorescent label to quench fluorescence from the fluorescent label,
• wherein the binding antibody specifically binds to RNase A and comprises a CDR3 selected from the group consisting of a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 5, a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 7.

An RNase detection immunoassay device, comprising:

• a substrate having a reaction space capable of accommodating a detection sample solution;
• a support located within the reaction space;
• a binding antibody coupled to the support;
• a switching peptide comprising a peptide compound reversibly bound to a Fab region of the binding antibody and a fluorescent label coupled to the peptide compound;
• and
• a quenching material disposed adjacent to the fluorescent label to quench fluorescence from the fluorescent label,
• wherein the binding antibody specifically binds to RNase A and comprises a CDR3 selected from the group consisting of a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 5, a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 7.

An RNase detection immunoassay method, comprising:

• a first step of fixing a binding antibody having a switching peptide coupled thereto to a substrate, or fixing the binding antibody to the substrate and then coupling the switching peptide to the binding antibody, and coupling a quenching material to the binding antibody or the substrate;
• a second step of treating the binding antibody with a detection sample solution;
• and
• a third step of, after the treating, irradiating the detection sample solution with light and quantitatively analyzing a target antigen in the detection sample solution via fluorescence generated from a fluorescent label of the switching peptide released from the binding antibody,
• wherein the binding antibody specifically binds to RNase A and comprises a CDR3 selected from the group consisting of a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 5, a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 7, and
• wherein the switching peptide comprises a peptide compound having an amino acid sequence capable of selectively and reversibly binding to a Fab (Fragment antigen-binding) region of the binding antibody, and a fluorescent label coupled thereto that emits fluorescence.

An RNase detection immunoassay method, comprising:

• a first step of fixing a binding antibody having a switching peptide coupled thereto to a support, or fixing the binding antibody to the support and then coupling the switching peptide to the binding antibody, and coupling a quenching material to the binding antibody or the support;
• a second step of injecting the support having the binding antibody coupled thereto and a detection sample solution into a reaction space of a substrate;
• and
• a third step of, after irradiating the detection sample solution with light, quantitatively analyzing a target antigen in the detection sample solution via fluorescence generated from a fluorescent label of the switching peptide released from the binding antibody,
• wherein the binding antibody specifically binds to RNase A and comprises a CDR3 selected from the group consisting of a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 5, a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 7, and
• wherein the switching peptide comprises a peptide compound having an amino acid sequence capable of selectively and reversibly binding to a Fab (Fragment antigen-binding) region of the binding antibody, and a fluorescent label coupled thereto that emits fluorescence.

A method for capturing RNase A, comprising:

• mixing an RNase A-containing sample with beads having an antibody or peptide that specifically binds to RNase A fixed to a surface thereof to induce a reaction between the RNase A and the antibody or peptide;
• and
• collecting the beads from the sample;
• wherein the binding antibody specifically binds to RNase A and comprises a CDR3 selected from the group consisting of a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 5, a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 7.