Engineered T Cells
AssigneeExpression Therapeutics LLC
InventorsHarrison BROWN, Brian PETRICH
Life of this patent
Estimating the term…
Abstract
A therapeutic molecule (single chain-based antibody or ligand-based) optimized for expression and secretion from engineered T cells, which may be gamma delta (gd) T cells. When expressed from engineered gdT cells, the STAR will be secreted and mediate engagement between gdT cells and antigen/receptor on target cells. Binding mediates the formation of a cytolytic synapse between the gdT cell and the target cell leading to activation the gdT cells to release proteolytic enzymes that kill target cells.
Claims15
A therapeutic agent comprising a single chain antibody variable domain that binds:
- PTK7, wherein the single chain antibody variable region that binds PTK7 has an amino acid sequence at least 96% identical to SEQ ID NO:
- 173, or
- SSTR2, wherein the single chain antibody variable region that binds SSTR-2 has an amino acid sequence at least 96% identical to SEQ ID NO:
- 181.
An engineered gamma delta T cell capable of secreting at least one therapeutic protein, the therapeutic protein comprising, from N-terminus to C-terminus, a gamma delta T cell optimized signal peptide, a tumor cell binding domain, a linker, and a T cell binding domain, wherein the tumor cell binding domain has an amino acid sequence at least 96% identical to SEQ ID NO:
- 173 or SEQ ID NO:
- 181.
A recombinant viral vector encoding a therapeutic protein capable of biosynthesis and secretion by a gamma delta T cell, the therapeutic protein comprising, from N-terminus to C-terminus, a gamma delta T cell optimized signal peptide that is cleaved off prior to secretion, a tumor cell-binding protein domain, a linker, and a T cell binding protein, wherein the tumor cell-binding protein domain binds:
- PTK7, wherein the single chain antibody variable region that binds PTK7 has an amino acid sequence at least 96% identical to SEQ ID NO:
- 173, or
- SSTR2, wherein the single chain antibody variable region that binds SSTR-2 has an amino acid sequence at least 96% identical to SEQ ID NO:
- 181.
Dependencies are inferred from the claim text.