Polypeptide with controlled association
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHikaru Koga, Shun Shimizu
Life of this patent
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Abstract
The present disclosure relates to polypeptides for which association between polypeptides is controlled, and in one embodiment, polypeptides for which association between Fc regions is controlled, methods for producing polypeptides for which association between Fc regions (in one embodiment, CH3 regions) is controlled, methods for controlling association between Fc regions, compositions comprising such an Fc region, compositions comprising a nucleic acid encoding such a polypeptide, and the like.
Claims28
A nucleic acid encoding a first polypeptide,
- wherein the first polypeptide comprises an Fc region into which a modification has been introduced,
- wherein, because of the modification introduced into the Fc region, the first polypeptide associates more readily with a first polypeptide having the modification via the Fc region than with a first polypeptide comprising an Fc region into which the modification has not been introduced.
A composition comprising a nucleic acid encoding a polypeptide,
- wherein the polypeptide comprises an Fc region into which a modification has been introduced,
- wherein the modification is at least one of the modifications set forth in Table 2.
A polypeptide which comprises an Fc region into which a modification has been introduced, wherein the polypeptide associates more readily with a polypeptide having the modification via the Fc region than with a polypeptide comprising an Fc region into which the modification has not been introduced due to at least one of the following actions resulting from the introduced modification:
- (1) steric complementarity, (2) disulfide linkage, and (3) electrostatic charge.
A method for obtaining a polypeptide for which association is controlled, comprising:
- obtaining a nucleic acid encoding the polypeptide;
- and
- expressing the nucleic acid,
- wherein the polypeptide comprises an Fc region, and
- wherein, because of the modification introduced into the Fc region, the polypeptide associates more readily with a polypeptide having the modification via the Fc region than with a polypeptide comprising an Fc region into which the modification has not been introduced.
A method for controlling association of a homomer of a polypeptide, comprising:
- obtaining a nucleic acid encoding the polypeptide;
- and
- expressing the nucleic acid,
- wherein the polypeptide comprises an Fc region, and
- wherein, because of the modification introduced into the Fc region, the polypeptide associates more readily with a polypeptide having the modification via the Fc region than with a polypeptide comprising an Fc region into which the modification has not been introduced.
A method for promoting expression of a homomer of a polypeptide, comprising:
- obtaining a nucleic acid encoding the polypeptide;
- and
- expressing the nucleic acid,
- wherein the polypeptide comprises an Fc region, and
- wherein, because of the modification introduced into the Fc region, the polypeptide associates more readily with a polypeptide having the modification via the Fc region than with a polypeptide comprising an Fc region into which the modification has not been introduced.
Dependencies are inferred from the claim text.