Artificial secretion peptides for heterologous protein production
AssigneeTenza, Inc.
InventorsAnik Debnath, Denise Sirias, Devon Stork
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Abstract
Provided herein are pre-protein signal peptides that direct secretion of expressed payload proteins from Lactobacillus bacteria. The pre-protein signal peptides described herein are designed for increased payload secretion over known signal peptides with minimal or reduced effect on bacterial strain fitness. Also provided herein are methods using the pre-protein signal peptides provided herein.
Claims49
A pre-protein signal peptide comprising an amino acid sequence that has at least 95%, 96%, 97%, 98%, 99%, or 100% identity to an amino acid sequence selected from SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 14, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 47, SEQ ID NO:
- 48, SEQ ID NO:
- 148, or SEQ ID NO:
- 180.
A polypeptide comprising a formula of X1-Z1 wherein:
- X1 is a pre-protein signal peptide;
- and
- Z1 is a payload protein.
A polypeptide comprising a formula of X1-Z1 wherein:
- X1 is a pre-protein signal peptide comprising the amino acid sequence of SEQ ID NO:
- 6, SEQ ID NO:
- 7, or SEQ ID NO:
- 14;
- and
- Z1 is a payload protein comprising an anti-TNFα antibody or an anti-TNFα antibody fragment.
A polypeptide comprising a formula of X1-Z1 wherein:
- X1 is a pre-protein signal peptide comprising the amino acid sequence of SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 14, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 47, SEQ ID NO:
- 48, SEQ ID NO:
- 148, or SEQ ID NO:
- 180;
- and
- Z1 is a payload protein comprising leptin.
A polypeptide comprising a formula of X1-Z1 wherein:
- X1 is a pre-protein signal peptide comprising the amino acid sequence of SEQ ID NO:
- 20;
- and
- Z1 is a payload protein comprising IL-10.
A polypeptide comprising a formula of X1-Z1 wherein:
- X1 is a pre-protein signal peptide comprising the amino acid sequence of SEQ ID NO:
- 7, SEQ ID NO:
- 14, SEQ ID NO:
- 148, or SEQ ID NO:
- 180;
- and
- Z1 is a payload protein comprising IL-22.
Dependencies are inferred from the claim text.