US 2026/0217796 A1Published application

Anti-aav2 monoclonal antibody, and preparation method and use thereof

OwnerLidan DING, Fei WANG +2inventors

InventorsLidan DING, Fei WANG, Long FAN, Yuzhuo HE

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Abstract

The invention belongs to the field of virus detection and diagnosis, and relates to an anti-AAV2 monoclonal antibody, and a preparation method and use thereof. Provided are an anti-AAV2 rabbit monoclonal antibody, and an amino acid sequence of a heavy chain variable region and a light chain variable region of the AAV2-resistant rabbit monoclonal antibody. The anti-AAV2 rabbit monoclonal antibody provided by the invention can be specifically combined with the VLP of the AAV2, and can be used for detecting an AAV virus antigen. The anti-AAV2 monoclonal antibody provided by the invention provides an effective detection tool for the detection of AAV2 in the research and development process of a gene therapy vector and the detection of AAV2 in basic science research.

Claims20

Dependent claims

An anti-AAV2 monoclonal antibody or a functional fragment thereof, wherein the antibody or the functional fragment thereof comprises heavy chain CDRs and light chain CDRs, the heavy chain CDRs and light chain CDRs are selected from the group consisting of CDRs with amino acid sequences as set forth in SEQ ID NO:

12 to SEQ ID NO:
35 and variants thereof each comprising at most three amino acid mutations, and the group is defined in terms of a combination pattern by one or more of groups a-d:
Heavy
Heavy
Heavy
Light
Light
Light
Combination
chain
chain
chain
chain
chain
chain
name
CDR1
CDR2
CDR3
CDR1
CDR2
CDR3
a
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
NO:
18 or
NO:
19 or
NO:
20 or
NO:
21 or
NO:
22 or
NO:
23 or
a variant
a variant
a variant
a variant
a variant
a variant
thereof
thereof
thereof
thereof
thereof
thereof
b
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
NO:
30 or
NO:
31 or
NO:
32 or
NO:
33 or
NO:
34 or
NO:
35 or
a variant
a variant
a variant
a variant
a variant
a variant
thereof
thereof
thereof
thereof
thereof
thereof
c
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
NO:
12 or
NO:
13 or
NO:
14 or
NO:
15 or
NO:
16 or
NO:
17 or
a variant
a variant
a variant
a variant
a variant
a variant
thereof
thereof
thereof
thereof
thereof
thereof
d
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
SEQ ID
NO:
24 or
NO:
25 or
NO:
26 or
NO:
27 or
NO:
28 or
NO:
29 or
a variant
a variant
a variant
a variant
a variant
a variant
thereof
thereof
thereof
thereof
thereof
thereof.

143 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/142,939Filed 26 Dec 2023
Priority: CN 202211676174.026 Dec 2022
Published: 30 Jul 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification17 codes

C07K 16/081
Against material from viruses: dNA viruses
C12N 7/02
Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof: recovery or purification
C12N 15/63
Recombinant DNA-technology: introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells