Modulating the properties of gpcr-based sensors via c-terminus isoforms
AssigneeGeorgia Tech Research Corporation
InventorsPamela Peralta-Yahya, Paola Lizeth Marquez-Gomez
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Abstract
G-protein coupled receptors (GPCRs) play a key role in chemical biosensing, detecting chemicals from odorants and hormones to neurotransmitters and peptides. GPCR-based sensors in yeast can be rapidly engineered by coupling human GPCRs to the yeast mating pathway, resulting in cell fluorescence or luminescence upon chemical detection. Modulating the properties of GPCR-based sensors including their dynamic and linear ranges is non-trivial, often requiring the engineering of the yeast cell machinery. Disclosed herein is the use of GPCR C-terminus isoforms and mutants to modulate the properties of chemical biosensors.
Claims18
A biosensor comprising:
- a sensing unit, the sensing unit comprising a G-protein coupled receptor (GPCR);
- wherein the GPCR has a truncated and/or mutated C-terminus;
- a synthetic processing unit, the synthetic processing unit comprising a signal transduction pathway comprising a synthetic transcription factor;
- a synthetic response unit, the synthetic response unit comprising a recombinant signal molecule gene, where the recombinant signal molecule gene is operatively coupled to a promoter that is responsive to the processing unit,
- wherein the sensing unit is in biologic communication with the processing unit and the processing unit is in biologic communication with the response unit.
An engineered yeast cell comprising:
- a sensing unit, the sensing unit comprising:
- a G-protein coupled receptor (GPCR);
- a processing unit, the processing unit comprising:
- a signal transduction pathway;
- a response unit, the response unit comprising:
- a recombinant signal molecule gene, where the recombinant signal molecule gene is operatively coupled to a promoter that is responsive to the processing unit,
- wherein the sensing unit is in biologic communication with the processing
- unit and the processing unit is in biologic communication with the response unit.
Dependencies are inferred from the claim text.