Barcoded xten polypeptides and compositions thereof, and methods for making and using the same
AssigneeAmunix Pharmaceuticals, Inc.
InventorsVolker Schellenberger, Eric Johansen, Angela Henkensiefken
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Abstract
Disclosed herein are polypeptides comprising an extended recombinant polypeptide (XTEN) comprised of a plurality of overlapping sequence motifs and one or more barcode fragments releasable upon protease digestion and detectable from all other proteolytically releasable fragments. Certain embodiments of these polypeptides further comprise a biologically active polypeptide, wherein advantageous embodiments thereof comprise a releasable segment capable of proteolytic cleavage that cleaves the linkage between the XTEN polypeptide and the biologically active polypeptide. Methods of making and methods of using said polypeptides are also disclosed.
Claims16
A polypeptide, comprising an extended recombinant polypeptide (XTEN) which:
- is from 150 to 1000 amino acids in length, at least 90% of which amino acids are glycine (G), alanine (A), serine (S), threonine (T), glutamate (E) or proline (P);
- comprises at least two times of a sequence motif selected from the group consisting of SEQ ID NOs:
- 182-203 and 1715-1722;
- and
- comprises a barcode fragment that occurs only once within the polypeptide and is located between 10 and 200 from the N-terminus or the C-terminus of the polypeptide,
- wherein the polypeptide further comprises a reference fragment that is further away, than the barcode fragment, from the N-terminus or the C-terminus of the polypeptide.
The polypeptide of 3, wherein the barcode fragment has an N-terminal amino acid that is immediately preceded by a glutamic acid residue.
Dependencies are inferred from the claim text.