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US 2026/0216683 A1Published application
Compartmentalized arrays, methods of preparing and uses thereof
AssigneeUniversity of Guelph
InventorsHuiyan Li, Roshan Aggarwal
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Abstract
The present application describes compartmentalized arrays, methods of preparing and uses thereof for multiplexed bioanalysis.
Claims20
A method of preparing a compartmentalized membrane array comprising:
- (a) adding one or more barriers on a surface of a membrane to form one or more compartments;
- (b) printing a hydrophilic polymer solution in the one or more compartments on the surface of the membrane in a plurality of distinct regions;
- (c) adding a solution comprising a blocking reagent to the one or more compartments, wherein the blocking reagent binds to the membrane surface in regions other than the plurality of distinct regions;
- and
- (d) washing the membrane to remove the hydrophilic polymer solution and excess blocking reagent, if present, to form the compartmentalized membrane array.
2 more elements
A method of preparing a reverse compartmentalized array comprising
- (a) coating a surface of a substrate with linker molecules to form a linker monolayer;
- (b) coating the linker monolayer with a blocking reagent solution to form a blocking reagent layer on the linker monolayer;
- (c) printing a solution of a blocking reagent remover on a plurality of distinct regions on the blocker reagent layer;
- and
- (d) washing the substrate to remove the blocking reagent remover and the blocking reagent on the plurality of distinct regions to form the reverse compartmentalized array,
- wherein the substrate comprises one or more barriers on the surface that form one or more compartments.
3 more elements
A reverse compartmentalized array comprising:
- (a) a substrate comprising a surface coated with a linker molecule monolayer;
- (b) a blocking reagent layer on the linker molecule monolayer;
- and
- (c) a plurality of distinct regions in the blocker reagent layer wherein the blocking reagent layer has been removed to expose the linker molecule monolayer;
- wherein the substrate comprises one or more barriers on the surface that form one or more compartments.
2 more elements
Dependencies are inferred from the claim text.