Production and use of bacterial cellulose in pure form or by impregnation of various agents and produced in spherical form for bone regeneration, alone and in combination with various graft materials
AssigneeAtaturk Universitesi RektöRlugu Bilimsel Arastirma Projeleri ( Bap ) Koordinasyon Birimi
InventorsSONGUL COMERT KILIC, MURAT OZDAL
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Abstract
Although this invention is primarily designed for various types of bone defects in dentistry, it can also be used in the medical field for various types of bone defects. It can be used in maxillary sinus augmentations, various intraosseous defect areas, and defect areas after intraosseous cyst surgeries, periimplantitis bone defects, and vertical/horizontal bone augmentations.
Claims9
Production of bacterial cellulose in pure form or by impregnation of various agents and produced in spherical form for bone regeneration, alone and in combination with various graft materials, characterized in that, it comprises the following process steps;
- in bacterial cellulose production, inoculating Komagataeibacter europaeus strain MOZ (GenBank:
- MW131623.1) bacteria into 250 mL flasks containing 50 ml broth (20 g/L mannitol, 4 g/L yeast extract, 8 g/L peptone, 2.3 g/L Na2HPO4, 1.0 g/L citric acid and pH 6.0), and for inoculum, growing bacteria grown in a liquid medium that does not contain citric acid, and using the prepared liquid culture for inoculation into 6% fermentation medium, and incubated for six days at 30° C. in a culture shaker incubator.
Production of bacterial cellulose in pure form or by impregnation of various agents and produced in spherical form for bone regeneration, alone and in combination with various graft materials, characterized in that, it comprises the following process steps of:
- purifying the bacterial cellulose at the end of the incubation, filtering the fermentation liquid and washing the same with distilled water, placing obtained celluloses in 0.1 M NaOH solution and keeping in a water bath at 90° C. for 30 minutes to remove bacterial cells and media residues, washing with distilled water until the pH reaches seven at the end of the period.
Use of bacterial cellulose obtained in pure form or by impregnation with various agents and produced in spherical form, characterized in that;
- it is the spherical preparation of bacterial cellulose in dispersed form and its use in various bone defects.
The use of bacterial cellulose obtained in pure form or by impregnation with various agents and produced in spherical form is characterized in that;
- it uses bacterial cellulose in various bone defects by preparing spherically dispersed cellulose and adding various bioactive agents therein.
Use of bacterial cellulose obtained in pure form or by impregnation with various agents and produced in spherical form, characterized in that;
- It is the use of bacterial cellulose in various bone defects by preparing the same spherically in dispersed form and mixing the same with various graft materials in particulate form.
Use of bacterial cellulose obtained in pure form or by impregnation with various agents and produced in spherical form, characterized in that;
- bacterial cellulose is prepared spherically in dispersed form and various bioactive agents are added therein, and this impregnated form is used in various bone defects by mixing the same with various graft materials in particulate form.
Dependencies are inferred from the claim text.