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US 2026/0216361 A1Published application
Nanobody-drug conjugates and methods of preparing thereof
AssigneeVanderbilt University
InventorsJohn T. Wilson, Karan Arora, Blaise R. Kimmel
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Abstract
Disclosed herein are nanobody-drug conjugates that can effectively deliver drugs, such as STING agonists. An example conjugate includes an albumin-binding nanobody, a drug, and a linker attaching the nanobody to the drug. Also disclosed are methods of making and using the conjugates.
Claims41
A conjugate comprising:
- a nanobody portion, the nanobody portion comprising an albumin-binding nanobody, wherein the albumin-binding nanobody is capable of specifically binding albumin;
- a drug;
- and
- a linker attaching the nanobody portion to the drug, the linker comprising a nanobody linking moiety attached to the nanobody portion, the nanobody linking moiety including an amino acid sequence of LPXT (SEQ ID NO:
- 1), wherein X is any amino acid.
2 more elements
A method of preparing a conjugate, the method comprising:
- reacting sortase with a nanobody portion, the nanobody portion including at least one nanobody having a sortase recognition site, to form a sortase-nanobody reagent, wherein the sortase-nanobody reagent comprises
- the nanobody portion,
- a nanobody linking moiety attached to the nanobody portion, the nanobody linking moiety including an amino acid sequence of LPXT (SEQ ID NO:
- 1), wherein X is any amino acid, and
- a sortase moiety attached to the nanobody linking moiety;
- reacting the sortase-nanobody reagent with a reactive amine reagent, the reactive amine reagent comprising a first reactive group, to form a reactive nanobody reagent, the reactive nanobody reagent comprising
- the nanobody portion,
- the nanobody linking moiety, and
- a first reactive group attached to the nanobody linking moiety;
- and
- coupling the reactive nanobody reagent with a reactive drug reagent, the reactive drug reagent comprising a drug and a second reactive group, to form a conjugate, the conjugate comprising
- the nanobody portion attached to the drug by a linker, the linker comprising the nanobody linking moiety.
9 more elements
Dependencies are inferred from the claim text.