Engineered cells and methods of use

Abstract

Aspects of the disclosure relate to a stromal cell comprising a heterologous nucleic acid encoding an ICAM1 protein. Further aspects relate to a solid support comprising a ICAM1 polypeptide linked to the solid support. Also provided is a a composition comprising (i) a stromal cell or solid support of the disclosure and (ii) pluripotent stem cell(s). Further aspects relate to a method for making a cell comprising culturing a composition of the disclosure under conditions suitable for the in vitro differentiation of the cell into a mature cell. Methods include a method for making a cell comprising culturing a composition described herein under conditions suitable for the in vitro differentiation of the cell into a mature cell. Cells made by the methods of the disclosure are also described as well as methods using the cells for treating a subject.

Dependencies are inferred from the claim text.