Methods of vaccine administration to salmonids

A method of protecting a salmonid against Piscine Myocarditis virus (PMCV) and at least one other pathogen, said method comprising administering to said salmonid a DNA vaccine comprising a nucleic acid sequence encoding SEQ ID NO:

• 3 or SEQ ID NO:
• 4 or an amino acid sequence at least 90% identical to SEQ ID NO:
• 3 or NO:
• 4 DNA vaccine comprising a nucleic acid sequence encoding SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or an amino acid sequence at least 90% identical to SEQ ID NO:
• 3 or NO:
• 4 wherein
• a) said DNA vaccine is injected intramuscularly
• b) a second vaccine is injected intraperitoneally substantially simultaneously with the DNA vaccine;
• c) said second vaccine comprises an antigen that elicits protective response against said at least one other pathogen;
• and
• d) said second vaccine is formulated as a W/O emulsion.