A wound dressing (40) comprising a support layer (1) adapted for enzymatic detection of an infection in a wound exudate, wherein the support layer (1) comprises a carrier material (10) that is capable of conducting liquids, the carrier material (10) has a first surface (11) and a second surface (12) opposite the first surface (11), and wherein the carrier material (10) comprises at least one test area (20) to which a test substance (21, 22) is applied, characterized in that the carrier material (10) comprises a diffusion barrier (30) which prevents lateral diffusion of the test substance (21, 22), such that the test substance (21, 22) is retained in the at least one test area (20) by the diffusion barrier (30).