Fusarium resistant lettuce plants
AssigneeNunhems B.V.
InventorsGowtham PRAKASH, Daan SCHREURS, Robert Johannes Martinus RAEDTS, Michiel VAN DEN MUNCKHOF, Bart GERAATS, Annick HOOGENDAM, Noor BREUGELMANS-DROUEN
Life of this patent
Estimating the term…
Abstract
The invention relates to a lettuce plant (Lactuca sativa L.) comprising a resistance QTL which confers dual resistance to Fusarium oxysporum f.sp lactucae race Fol-1 and race Fol-4, wherein the QTL is located on chromosome 7. The invention further provides seeds, progeny, propagation material and food products from the plant.
Claims12
A lettuce plant (Lactuca sativa L.) comprising a Quantitative Trait Locus (QTL) named QTL7.1 from Lactuca serriola, which confers resistance to Fusarium oxysporum f.sp. lactucae races Fol-1 and Fol-4, wherein the QTL7.1 is located on chromosome 7 between nucleotide 64.382.881 (SNP_01 at nucleotide 101 of SEQ ID NO:
- 1) and nucleotide 69.375.685 (SNP_10 at nucleotide 101 of SEQ ID NO:
- 10) and wherein said QTL7.1 is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB 44108.
A method for detecting or selecting a plant or plant part or seed comprising QTL7.1, said method comprising determining the presence in the genome of the plant, plant part or seed of at least 2, 3, 4, 5, 6, 7 or 8 of the following SNP markers:
- a Guanine (G) for SNP_02 at nucleotide 101 of SEQ ID NO:
- 2 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 2,
- a Cytosine (C) for SNP_03 at nucleotide 101 of SEQ ID NO:
- 3 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 3,
- an Adenine (A) for SNP_04 at nucleotide 101 of SEQ ID NO:
- 4 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 4,
- a Guanine (G) for SNP_05 at nucleotide 101 of SEQ ID NO:
- 5 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 5,
- a Guanine (G) for SNP_06 at nucleotide 101 of SEQ ID NO:
- 6 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 6,
- a Thymine (T) for SNP_07 at nucleotide 101 of SEQ ID NO:
- 7 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 7,
- a Thymine (T) for SNP_08 at nucleotide 101 of SEQ ID NO:
- 8 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 8, and/or
- an Adenine (A) for SNP_09 at nucleotide 101 of SEQ ID NO:
- 9 or at the equivalent nucleotide of a sequence comprising at least 95% sequence identity to SEQ ID NO:
- 9,
- and optionally selecting a plant, or plant part or seed comprising said SNP markers.
A method of producing an L. sativa plant comprising an introgression fragment on chromosome 7 comprising QTL7.1 comprising:
- crossing a first lettuce (L. sativa) plant comprising a recombinant chromosome 7 in homozygous form having an introgression fragment comprising QTL7.1 and comprising the donor SNP nucleotide for at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 of the SNP markers linked to the QTL, with a second lettuce (L. sativa) plant.
Dependencies are inferred from the claim text.