AcTEAvator System
AssigneeThe Regents of the University of California
InventorsJacquelyn Gervay-Hague, David M. Tricoli
Life of this patent
Estimating the term…
Abstract
Provided herein are methods and kits for propagation of tea plant varieties that can reduce the time to harvest from about 7 years to about 5 months by using a sequence of media formulations comprising gibberellic acid (GA3), indole-3-butyric acid (IBA), and at least one of benzylaminopurine (BAP) and meta-Topolin (mT).
Claims57
A method of propagating a Camellia plant comprising:
- (i) placing the base of a shoot cutting from a Camellia plant in a budding medium for a sufficient length of time until the shoot cutting has at least one bud that has elongated to at least 1 cm,
- wherein the budding medium comprises Murashige and Skoog medium (MS medium) with half-strength macronutrients and full-strength micronutrients or its equivalent, a gibberellin, an auxin, and a cytokinin;
- (ii) transferring the shoot cutting with at least one bud to a shoot multiplication medium for a sufficient length of time until the shoot cutting has at least two offshoots,
- wherein the shoot multiplication medium comprises Driver and Kuniyuki walnut minimal organics medium (DKW medium), a gibberellin, an auxin, and a cytokinin;
- and
- (iii) transferring the shoot cutting with at least two offshoots to a shoot elongation medium comprising DKW medium, a gibberellin, an auxin, and a cytokinin.
A kit for propagation of a tea plant comprising:
- a budding medium comprising ascorbic acid at a concentration in the range of 0 to 500 mg/L, citric acid at a concentration in the range of 0 to 500 mg/L, L-cysteine at a concentration in the range of 0 to 500 mg/L, reduced glutathione at a concentration in the range of 0 to 200 mg/L, glutamine at a concentration in the range of 0 to 500 mg/L, putrescine at a concentration in the range of 0.1 to 10 mM, ferrous sulfate at a concentration in the range of 0 to 250 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, IBA at a concentration in the range of 0 to 10 mg/L, and PPM™ at a concentration in the range of 0 to 10 mL/L;
- a shoot multiplication medium comprising DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, and IBA at a concentration in the range of 0 to 10 mg/L;
- a shoot elongation medium comprising DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L or mT at a concentration in the range of 0.1 to 10 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, and IBA at a concentration in the range of 0 to 10 mg/L;
- and
- an optimized culture medium comprising DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, mT at a concentration in the range of 0.1 to 10 mg/L, GA3 at a concentration in the range of 0 to 50 mg/L, IBA at a concentration in the range of 0 to 1 mg/L, calcium gluconate at a concentration in the range of 0.1 to 10 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, sodium thiosulfate and sodium nitrate at a concentration in the range of 5 to 20 mM.
A composition comprising a budding medium for propagation of a tea plant, wherein the budding medium comprises ascorbic acid at a concentration in the range of 0 to 500 mg/L, citric acid at a concentration in the range of 0 to 500 mg/L, L-cysteine at a concentration in the range of 0 to 500 mg/L, reduced glutathione at a concentration in the range of 0 to 200 mg/L, glutamine at a concentration in the range of 0 to 500 mg/L, putrescine at a concentration in the range of 0.1 to 10 mM, ferrous sulfate at a concentration in the range of 0 to 250 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, IBA at a concentration in the range of 0 to 10 mg/L, and PPM™ at a concentration in the range of 0 to 10 mL/L.
A composition comprising a shoot multiplication medium for propagation of a tea plant, wherein the shoot multiplication medium comprises DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, and IBA at a concentration in the range of 0 to 10 mg/L.
A composition comprising a shoot elongation medium for propagation of a tea plant, wherein the shoot elongation medium comprises DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, BAP at a concentration in the range of 0.1 to 50 mg/L or mT at a concentration in the range of 0.1 to 10 mg/L, GA3 at a concentration in the range of 0 to 40 mg/L, and IBA at a concentration in the range of 0 to 10 mg/L.
A composition comprising an optimized culture medium for propagation of a tea plant, wherein the optimized culture medium comprises DKW medium, sucrose at a concentration in the range of 10 to 50 g/L, mT at a concentration in the range of 0.1 to 10 mg/L, GA3 at a concentration in the range of 0 to 50 mg/L, IBA at a concentration in the range of 0 to 1 mg/L, calcium gluconate at a concentration in the range of 0.1 to 10 g/L, putrescine at a concentration in the range of 0.1 to 10 mM, glutamine at a concentration in the range of 0 to 500 mg/L, sodium thiosulfate and sodium nitrate at a concentration in the range of 5 to 20 mM.
Dependencies are inferred from the claim text.