Calibration
AssigneeBelgian Volition SRL
InventorsMark Edward Eccleston, Theresa K. Kelly
Life of this patent
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Abstract
The invention relates to methods for quantifying the amount of a nucleosome or a histone associated with a nucleosome in a biological sample wherein the method is calibrated using a nucleosome reference standard in a pre-calibration step.
Claims27
A method for quantifying an amount of a nucleosome or a histone associated with a nucleosome in a biological sample, the method comprising:
- (i) providing a sample of nucleosomes at various concentrations to create a reference standard wherein the nucleosomes are assayed to generate a standard curve in a pre-calibration step;
- (ii) adding a binding agent to a biological sample to perform an assay to measure an amount of nucleosome or histone associated with the nucleosome in the biological sample;
- and
- (iii) quantifying the amount of nucleosome or histone associated with the nucleosome in the biological sample by measuring the amount of binding agent bound in the biological sample and comparing the amount to the reference standard.
A method for measuring a level of nucleosomes or histone associated with nucleosomes in a biological sample, the method comprising:
- (i) obtaining a sample of a biological fluid;
- (ii) contacting the sample with a binding agent to the nucleosomes;
- (iii) measuring a parameter or degree of nucleosome binding of the binding agent to the nucleosome using an instrument;
- (iv) comparing the parameter or degree of nucleosome binding obtained in step (iii) with standardisation data;
- and
- (v) using the comparison in step (iv) to convert the parameter or degree of nucleosome binding measured in step (iii) into an amount or concentration of nucleosome present in the sample.
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A kit for measuring the level of nucleosomes or histone associated with nucleosomes in a biological sample or for quantifying an amount of a nucleosome or a histone associated with a nucleosome in a biological sample, wherein the kit comprises an automated immunoassay analyser, a nucleosome binder and electronically accessible standardisation data and does not comprise a nucleosome standard or calibrant.
Dependencies are inferred from the claim text.