Identification and monitoring of monoclonal immunoglobulins by molecular mass
AssigneeMayo Foundation for Medical Education and Research
InventorsDavid R. Barnidge, David L. Murray
Life of this patent
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Abstract
Disclosure herein are methods for determining whether or not an immunoglobulin is present above the polyclonal background level in a biological sample, and methods for determining whether an immunoglobulin contains a kappa or lambda light chain. These methods are useful for screening biological samples for the presence or absence of monoclonal gammopathy, and for diagnosing and monitoring monoclonal gammopathy in a subject.
Claims23
1-30. (canceled)
A method of detecting a monoclonal kappa or lambda light chain immunoglobulin expansion in a sample, comprising:
- subjecting a sample comprising intact immunoglobulin kappa or lambda light chains to tandem mass spectrometry;
- and
- detecting the presence of the kappa or lambda light chain immunoglobulin expansion by measuring constant region fragment ions produced by collision induced dissociation.
A method for determining whether a monoclonal light chain immunoglobulin is a kappa light chain or a lambda light chain, comprising:
- subjecting a sample comprising intact immunoglobulin light chains to tandem mass spectrometry;
- and
- comparing m/z's of one or more fragment ions produced by collision induced dissociation to m/z's that are expected to result from the constant region of the kappa light chain or the lambda light chain
Dependencies are inferred from the claim text.