Multiparametric integrated molecular detection of viral antigens and viral nucleic acids
AssigneeOhio State Innovation Foundation
InventorsEduardo REATEGUI, Truc Kim NGUYEN
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Abstract
Disclosed herein are methods for detecting and treating viral infections. Disclosed is a method of detecting a virus, comprising obtaining a biological sample; capturing a plurality of membranous particles from the biological sample; measuring antigens and nucleic acid levels in the membranous particles or single virions from the biological sample; and measuring an amount of a viral RNA; wherein a virus is detected when the viral protein level or the amount of viral RNA is increased in comparison to a control sample.
Claims18
A method of detecting a viral antigen and a viral nucleic acid in a subject, comprising:
- obtaining a biological sample from the subject;
- capturing a virion from the biological sample;
- or
- capturing a membranous particle from the biological sample;
- and
- measuring the viral antigen and the viral nucleic acid level in the biological sample;
- wherein the virion or membranous particle is immobilized on a biochip.
A method of treating a viral infection in a subject, comprising:
- obtaining a biological sample from the subject;
- capturing a virion from the biological sample;
- or
- capturing a membranous particle from the biological sample;
- measuring a viral antigen or a viral nucleic acid level in the biological sample;
- wherein the virion or membranous particle is immobilized on a biochip;
- and
- administering an antiviral agent if a viral infection is detected.
A method for high throughput multiplexing for simultaneous detection of a viral antigen and a viral nucleic acid, the method comprising the steps of:
- immobilizing a virion and/or a membranous particle on a plasmonic surface;
- detecting the viral antigen and the viral nucleic acid simultaneously derived from the virion and/or membranous particle;
- wherein the detection method comprises fluorescence, in situ hybridization, enzyme linked immunosorbent assay, flow cytometry, or microscopy.
Dependencies are inferred from the claim text.