Reagent matrix comprising cellulose nanofibrils and its use for detecting analytes

A kit for performing an assay for detection of an analyte in a sample, the kit comprising:

• a reagent matrix, comprising;
• a plurality of a first capture component comprising a first analyte capture site;
• a plurality of a second capture component comprising a second analyte capture site, and at least one of a cellulose binding domain (CBD) or a carbohydrate binding module (CBM), wherein at least one of the first capture component or the second capture components comprises a detection medium;
• and
• a plurality of cellulose nanofibrils;
• and
• a porous substrate.

A method for detection of an analyte in a sample, the method comprising:

• combining the sample, a reagent matrix, and a carrier solution in a vessel to form a testing sample, wherein the reagent matrix comprises;
• a plurality of a first capture component comprising a first analyte capture site;
• a plurality of a second capture component comprising a second analyte capture site, and at least one of a cellulose binding domain (CBD) or a carbohydrate binding module (CBM), wherein at least one of the first capture component or the second capture components comprises a detection medium;
• and
• a plurality of cellulose nanofibrils;
• and
• contacting the testing sample to a porous substrate;
• and
• detecting a signal produced by an aggregation of the analyte with the reagent matrix on a surface of the porous substrate.