A personalized cosmetic recommendation method based on immunochromatographically characterizing, simultaneously and immediately after sampling, different biomarkers of the skin-microbiota complex of an individual comprising the following steps:
- providing a sample of biological material taken from the surface of the skin and containing the constituent elements of the skin-microbiota complex, the skin-microbiota complex consisting of skin cells and microorganisms and other constituent elements of the skin microbiota;
- suspending the skin-microbiota complex in a solubilization solution;
- and
- pouring the solubilization solution into the transfer well of an immunochromatographic revealing device, the immunochromatographic revealing device comprising a support on which is arranged a transfer well configured to receive the solubilization solution, the well leading to a plurality of N revealing zones each having (i) an immunological detection reagent and (ii) an immunological capture reagent, and, the immunological revealing device comprising:
- at least one immunological detection reagent specific for a skin cell biomarker and at least one immunological detection reagent specific to a biomarker of the skin microbiota;
- the immunological detection reagents each comprise an antibody specific to a biomarker of skin cells or of the skin microbiota, conjugated to a colorimetric identification system;
- the immunological capture reagents each comprise an antibody, bound to the support in each of the N revealing zones, capable of recognizing the biomarker present in the zone;
- and
- at least one fool-proofing means for orienting the reading of the device;
- incubating between 1 and 10 min in order to allow the cells of the skin-microbiota complex to react with the reagents on each of the N zones of the immunological revealing device;
- processing the image consisting of the set of signals revealed on each of the N revealing zones via a computer enabling the skin-microbiota complex to be categorized by processing this combination of signals;
- and
- recommending one or more cosmetic products based on the combination of biomarkers revealed;
- wherein:
- the number N of revealing zones is greater than or equal to 5 and comprises a) at least three zones for revealing three biomarkers of the skin microbiota, the biomarkers being bacteria of the genera Staphylococcus, Cutibacterium and Corynebacterium and b) at least one zone for revealing a skin cell biomarker selected from (i) a structural protein, (ii) an inflammatory biomarker or (iii) an allergy biomarker;
- and
- the recommended cosmetic products are suited on the one hand to the skin type of the individual and are, on the other hand, compatible with the microbiota thereof.