A method of producing biomolecule-crosslinked compound and a compound derived thereof

A method of producing a biomolecule-crosslinked compound comprising the steps of:

• reacting a plurality of monomers and a plurality of comonomers in the presence of ammonia within a first solution to generate a compound;
• retrieving the produced compound from the first solution for cleaning;
• subjecting the cleaned compound to react with a second solution to join a crosslinker onto the compound through a first amine functional group of the crosslinker;
• and
• adding a plurality of biomolecules to the second solution to bind the biomolecules towards the compound through a second amine functional group of the crosslinker to produce the biomolecule-crosslinked compound,
• wherein the monomers are Tetrapropyl orthosilicate (TPOS), Tetraethyl orthosilicate (TEOS), Tetramethyl orthosilicate (TMOS), Tetra-isopropyl orthosilicate or Butyl orthosilicate, wherein the comonomers are selected from (3-Aminopropyl)-triethoxysilane), (3-Aminopropyl)-trimethoxysilane), (3-Aminopropyl-methyl-diethoxysilane), (3-(Dimethoxymethylsilyl) propylamine), (3-Aminopropylsilanetriol), (N-(2-Aminoethyl) (3-aminopropyl)methyldimethoxysilane) or N-(2-Aminoethyl) (3-aminopropyl) trimethoxysilane).

A method of producing amine-functionalized silica nanoparticles (AFSNPs) comprising:

• reacting a plurality of monomers and a plurality of comonomers in the presence of ammonia within a first solution to generate the AFSNPs;
• and
• retrieving the produced AFSNPs from the first solution for cleaning,
• wherein the monomers are Tetrapropyl orthosilicate (TPOS), Tetraethyl orthosilicate (TEOS), Tetramethyl orthosilicate (TMOS), Tetra-isopropyl orthosilicate or Butyl orthosilicate, and the comonomers are selected from (3-Aminopropyl)-triethoxysilane), (3-Aminopropyl)-trimethoxysilane), (3-Aminopropyl-methyl-diethoxysilane), (3-(Dimethoxymethylsilyl) propylamine), (3-Aminopropylsilanetriol), (N-(2-Aminoethyl) (3-aminopropyl)methyldimethoxysilane) or N-(2-Aminoethyl) (3-aminopropyl) trimethoxysilane).