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US 2026/0210951 A1Published application
Compositions, Methods, and Kits for the Isolation of Extracellular Vesicles
AssigneeExosome Diagnostics, Inc.
InventorsJohan Karl Olov Skog, Olubode A. Ogunlusi, Robert Raymond Kitchen, Douglas Roberts, Wei Yu
Life of this patent
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Abstract
The present disclosure relates to compositions, methods and kits for the isolation of extracellular vesicles. The compositions, methods and kits can comprise a high-density liquid reagent that facilitates the sequestration of extracellular vesicles bound to magnetic beads.
Claims21
1-45. (canceled)
A method of isolating a microvesicle comprising a surface marker from a biological sample, the method comprising:
- a) incubating the biological sample with at least one particle that binds the surface marker under conditions sufficient to form a particle-microvesicle complex;
- b) contacting the biological sample comprising the particle-microvesicle complex with a high-density matrix;
- and
- c) subjecting the biological sample comprising the particle-microvesicle complex and the high-density matrix to a force such that the particle-microvesicle complex is translocated through the high-density matrix, thereby isolating the microvesicle.
1 more element
A kit for isolating a microvesicle comprising:
- a) a plurality of particles that bind a surface marker on the microvesicle;
- b) a high-density matrix;
- and
- c) instructions for isolating the microvesicle from a biological sample, wherein the instructions indicate contacting the biological sample with the high-density matrix at a ratio from about 1:1 to about 1:100 (vol/vol) of the biological sample to the high-density matrix.
1 more element
Dependencies are inferred from the claim text.