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US 2026/0210944 A1Published application
Methods, culture medias and devices for generating embryos in vitro from stem cells
AssigneesCalifornia Institute of Technology, Cambridge Enterprise Limited
InventorsMagdalena D. Zernicka-Goetz, Gianluca Amadei, Charlotte Handford
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Abstract
Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.
Claims21
A method of generating a synthetic embryo in vitro, the method comprising:
- culturing a post-implantation embryo structure in a post-implantation culture media under a static condition allowing the post-implantation embryo structure to develop into a neurulating embryo structure, wherein the post-implantation culture media comprises a basal culture medium, human cord serum, and bicarbonate or HEPES;
- and
- culturing the neurulating embryo structure for at least one day in the post-implantation culture media under a dynamic condition in a culture chamber allowing the neurulating embryo structure to develop into a synthetic embryo of at least early organogenesis stage.
Dependencies are inferred from the claim text.