US 2026/0210918 A1Published application

Method for simultaneously detecting four alkaloids and 10 catechin components in tea

AssigneeTea Research Institute, Guangdong Academy of Agricultural Sciences

InventorsDandan Qin, Hualing Wu, Kaixing Fang, Erdong Ni, Qiushuang Wang, Qing Wang, Hongjian Li, Bo Li, Chendong Pan

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present disclosure belongs to the technical field of tea component detection methods, and specifically relates to a method for simultaneously detecting four alkaloids and 10 catechin components in tea. The method includes: simultaneously detecting four alkaloids and 10 catechin components in a tea sample by using HPLC, and obtaining the content of the 14 characteristic components in the tea sample according to respective standard curves of the four alkaloids and 10 catechin components. The detection method provided by the present disclosure enables simultaneous detection and separation of 14 compounds in tea, which solves the problem of poor separation effect when simultaneously analyzing multiple compounds in tea in the prior art, meets high-standard requirements for tea component analysis, provides a more efficient, accurate and reliable analysis tool for tea producers, testing institutions and researchers, and provides strong support for tea quality control, scientific research, deep processing, and other fields.

Claims11

Dependent claims

A method for simultaneously detecting four alkaloids and 10 catechin components in tea, comprising the following steps:

simultaneously detecting four alkaloids and 10 catechin components in a tea sample by using high-performance liquid chromatography, and obtaining content of the 14 characteristic components in the tea sample according to respective standard curves of the four alkaloids and 10 catechin components;
chromatographic conditions are as follows:
a chromatographic column is Agilent Zorbax Eclipse Plus C18, a column temperature is 30-40° C., a mobile phase C is 100% acetonitrile, a mobile phase D is a 0.05% phosphoric acid aqueous solution, a flow rate is 0.9-1.1 mL/min, and a detection wavelength is 275-285 nm;
and performing quantification based on a peak area according to an external standard method, wherein a gradient elution program is as follows:
Time/min
Mobile phase C/%
Mobile phase D/%
0.01
6.5
93.5
30
16
84
34
20
80
39
20
80
40
35
65
45
35
65
50
6.5
93.5
60
End
End
wherein the four alkaloids are theophylline, theobromine, caffeine, and theacrine;
and the 10 catechins are epicatechin, catechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, catechin gallate, gallocatechin gallate, gallocatechin, epigallocatechin-3-O-(3-O-methyl) gallate, and theaflavin.

30 more elements

Dependencies are inferred from the claim text.