Polarized fourier light-field imaging
AssigneeCalifornia Institute of Technology
InventorsOumeng Zhang, Changhuei Yang
Life of this patent
Estimating the term…
Abstract
Polarized Fourier light field imaging that involve a microlens array segmenting fluorescence emissions at different angles, an optical configuration that converts the fluorescence emissions into spatial frequencies in the Fourier domain, and a polarization sensor that can acquire a raw image including polarized sub-images corresponding to polarization channels.
Claims20
A polarized Fourier light-field imaging system, comprising:
- a first optical system in optical communication with one or more light sources, the first optical system configured to deliver polarized excitation illumination to a sample being imaged, the sample issuing fluorescence emissions induced by the polarized excitation illumination, the first optical system comprising a microlens array at a pupil plane, the microlens array comprising a plurality of lenslets configured to segment fluorescence emissions issuing from the sample from different angles;
- a second optical system in optical communication with the microlens array, the second optical system configured to convert the fluorescence emissions into spatial frequencies in the Fourier domain;
- and
- a polarization sensor in optical communication with the second optical system, the polarization sensor configured to acquire one or more raw images, each raw image including a plurality of polarized sub-images corresponding to a respective plurality of polarization channels.
A polarized Fourier light-field imaging method, comprising:
- (a) obtaining a raw image of a sample using a polarization camera, the raw image including a plurality of polarized two-dimensional (2D) sub-images corresponding to a plurality of polarization channels associated with polarization angles of polarized pixels of the polarization camera, the raw image acquired based on fluorescence emissions emitted from fluorophores in the sample in response to activation by polarized excitation illumination;
- (b) applying a 2D-to-3D reconstruction procedure to the plurality of 2D polarized sub-images to generate a corresponding plurality of three-dimensional (3D) volumetric datasets;
- and
- (c) applying voxel-wise combination of the plurality of 3D volumetric datasets based on Stokes relationships to generate a volumetric fluorescence image.
A non-transitory machine-readable medium comprising instructions that, when executed by one or more processors, are configured to cause the one or more processors to perform operations comprising:
- (a) obtaining a raw image of a sample using a polarization camera, the raw image including a plurality of polarized two-dimensional (2D) sub-images corresponding to a plurality of polarization channels associated with polarization angles of polarized pixels of the polarization camera, the raw image acquired based on fluorescence emissions emitted from fluorophores in the sample in response to activation by polarized excitation illumination;
- (b) applying a 2D-to-3D reconstruction procedure to the plurality of polarized 2D sub-images to generate a corresponding plurality of three-dimensional (3D) volumetric datasets;
- and
- (c) applying voxel-wise combination of the plurality of 3D volumetric datasets based on Stokes relationships to generate a volumetric fluorescence image.
Dependencies are inferred from the claim text.