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US 2026/0209833 A1Published application
Methods, compositions, and kits for detecting spatial chromosomal interactions
Assignee10x Genomics, Inc.
InventorLayla Katiraee
Life of this patent
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Abstract
The present disclosure features methods, compositions, and kits for spatially determining the location of chromosomal conformation interactions in a biological sample.
Claims20
A method for analyzing chromosomal conformation interactions in a biological sample, the method comprising:
- (a) providing an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises:
- (i) a spatial barcode and (ii) a capture domain;
- (b) crosslinking accessible DNA in the biological sample;
- (c) fragmenting the crosslinked accessible DNA, thereby generating crosslinked fragmented DNA;
- (d) circularizing the crosslinked fragmented DNA, thereby generating crosslinked circularized DNA;
- (e) digesting the crosslinked circularized DNA, thereby generating crosslinked digested DNA;
- (f) incorporating a capture sequence onto an end of the crosslinked digested DNA;
- and
- (g) hybridizing the capture sequence of the crosslinked digested DNA to the capture domain of the capture probe on the array.
6 more elements
Dependencies are inferred from the claim text.