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US 2026/0209828 A1Published application
Reagents and methods for normalization
AssigneeTwist Bioscience Corporation
InventorsSiyuan CHEN, Ramsey Ibrahim ZEITOUN, Xuan Yu Elian LEE, Steve Dojin OH, Kristin Deann BUTCHER, Owen Kabnick SMITH
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Abstract
Provided herein are compositions and methods for normalizing sequencing libraries. Further provided herein are adapter conjugates and hybrid circular adapters, composition comprising the same, and methods of generating the same. Further provided herein are methods of using the adapter conjugates, hybrid circular adapters, and compositions comprising the same to normalize genomic DNA libraries for next-generation sequencing.
Claims22
A composition comprising:
- a first polynucleotide adapter comprising:
- a first strand, wherein the first strand comprises a first terminal adapter region, a first non-complementary region, and a first yoke region;
- a second strand, wherein the second strand comprises a second terminal adapter region, a second non-complementary region, and a second yoke region;
- wherein the first yoke region and the second yoke region are complementary, wherein the first non-complementary region and the second non-complementary region are not complementary,
- a second polynucleotide adapter comprising:
- a third strand, wherein the third strand comprises a third terminal adapter region, a third non-complementary region, and a third yoke region;
- and
- a fourth strand, wherein the fourth strand comprises a fourth terminal adapter region, a fourth non-complementary region, and a fourth yoke region,
- wherein the third yoke region and the fourth yoke region are complementary, and wherein the third non-complementary region and the fourth non-complementary region are not complementary, and
- wherein the first polynucleotide adapter and the second polynucleotide adapter are connected via a linker.
7 more elements
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Dependencies are inferred from the claim text.