Materials and methods for characterizing potency
AssigneeEncoded Therapeutics, Inc.
InventorsAnne Tanenhaus, Sirika Wood, Chi Zhang, Dhruv Bole, Tiffany Cai, Steven Tan
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Abstract
The disclosure provides a nucleic acid comprising a response element operably linked to a reporter nucleotide sequence. In various embodiments, the response element comprises from 2 to 10 copies of a Z1 transcription factor binding site. Cells comprising the nucleic acid also are provided. In various aspects, the cells stably overexpress an adeno-associated virus receptor. The disclosure further provides a method of determining the potency of a sample comprising AAV vectors.
Claims87
A nucleic acid comprising a response element operably linked to a reporter nucleotide sequence, wherein the response element comprises from 2 to 10 copies of a Z1 transcriptional factor (TF) binding site.
A cell comprising a nucleic acid comprising a response element operably linked to a reporter nucleotide sequence, wherein the cell is further engineered to stably overexpress an adeno-associated virus receptor (AAVR).
A nucleic acid comprising a response element operably linked to a reporter nucleotide sequence, wherein the response element comprises a transcriptional factor (TF) binding site bound by an endogenous metal-dependent TF.
The nucleic acid of any one of claims 61-65, wherein the response element further comprises a promoter.
Dependencies are inferred from the claim text.