Trigger-inducible mrna circularization
AssigneeWestlake University
InventorsJiawei SHAO, Shichao LI, Hui WANG, Xinyuan QIU, Mingqi XIE
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Abstract
Provided is a gene regulation strategy enabling programmable control over eukaryotic translational initiation and use of said gene regulation strategy for various biomedical purposes including but not limited to therapeutic transgene delivery, intracellular sensing, molecular diagnostics and gene- and cell-based therapies. Also provided is a method to detect and eliminate cancer cells harboring fusion proteins
Claims34
A gene regulation system that can express the target gene mRNA by regulation, which comprises
- (i) Synthetic translation initiation Factors (STIFs), and an mRNA construct that comprises the mRNA encoding the target protein;
- or
- (ii) An nucleic acid that encodes the STIF(s) and a nucleic acid that encodes the mRNA construct that comprises the mRNA encoding the target protein, e.g., said nucleic acid is DNA or RNA.
- wherein the STIF comprises or consists of at least one eIFBP (eIF4F-binding proteins), and at least one RBP (RNA binding proteins).
The system of claim 25, wherein the mRNA construct can further comprise the cleavage as defined in anyone of claims 19-22.
Dependencies are inferred from the claim text.