Production of cannabinoids and engineered cells therefor
AssigneeCellibre, Inc.
InventorsDiep Minh Ngoc Nguyen, Caleb Marshall Walker, Nicky Christopher Caiazza, Jun Urano
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Abstract
Disclosed herein are novel cells which are engineered to catabolize a reduced number of acyl-CoA and branched chain amino acids and methods for improved cannabinoid production using these cells. Additionally, cells which are engineered to produce rare cannabinoids, methods for producing these novel rare cannabinoids, as well as the novel rare cannabinoids compounds thus produced, are also disclosed.
Claims20
-39. (canceled)
A cell engineered to provide increased production of cannabinoids and/or derivatives thereof compared to a control cell, the engineered cell expressing one or more enzymes of the cannabinoid biosynthesis pathway,
- wherein at least one gene encoding one or more enzymes or other proteins influencing metabolism of at least one acyl-CoA molecule of the cell has been deleted, inactivated, or otherwise mutated, thereby permitting the redirection of acyl-CoA molecules from a cellular metabolic pathway to a cannabinoid biosynthesis pathway to achieve increased production of cannabinoids and/or derivatives thereof compared to a control cell, and
- wherein the control cell is lacking the same deletion, inactivation or other mutation of the at least one gene as the engineered cell.
A cell engineered to produce one or more of CBGiBA, CBGA-uns, CBGA-keto, THCiBA, THCA-uns, THCA-keto, CBCiBA. CBCA-uns, CBCA-keto, CBDiBA, CBD-uns, CBD-keto, and/or decarboxylated derivatives thereof, wherein the cell comprises an inactivated or otherwise mutated IVD1, POT1, and/or MFE1 gene, and wherein the cell further expresses sufficient enzymes in the cannabinoid synthesis pathway to produce the one or more of CBGiBA, CBGA-uns, CBGA-keto, THCiBA, THCA-uns, THCA-keto, CBCiBA. CBCA-uns, CBCA-keto, CBDiBA, CBD-uns, CBD-keto, and/or decarboxylated derivatives thereof, when cultured under suitable conditions, optionally, wherein the suitable conditions comprise supplementation of a culture media in which the cell is cultured with butyric acid, butyl-butyrate, valeric acid, isovaleric acid, butanol, hexanol, hexanoic acid, hexyl-hexanoate, oleic acid, glycerol or glucose.
A compound according to any of the following structures:
Dependencies are inferred from the claim text.