EvolvR Polypeptides and Methods of Use Thereof

Abstract

The present disclosure provides an EvolvR polypeptides. An EvolvR polypeptide of the present disclosure (i.e., a subject EvolvR polypeptide) comprises: (a) an enzymatically active RNA-guided endonuclease (CRISPR-Cas effector protein such as a Slug-nCas9) that introduces a single-stranded break in a target DNA (i.e., is a nickase); and (b) an error-prone DNA polymerase. In some cases, (a) is fused to (b). In some cases, (a) and (b) are fused to first and second members, respectively, of a dimerization pair, and there (a) and (b) are brought together upon dimerization. In some cases the dimerization is constitutive and in some cases the dimerization is inducible (e.g., using a dimerizing agent).

Dependencies are inferred from the claim text.