Aminoacyl-trna synthetases and uses hereof
AssigneeNovo Nordisk A/S
InventorHongtao Guan
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Abstract
The invention relates to aminoacyl-tRNA synthetases that aminoacylate tRNA with 2-Aminoisobutyric acid (Aib), thus enabling the incorporation of the Aib into a growing polypeptide chain during translation, e.g. in eubacterial host cells such as E. coli. For example, but not limited to, the invention relates to new aminoacyl-tRNA synthetases and the uses hereof, as well as a method for producing polypeptides that contain one or more Aib.
Claims6
-14. (canceled)
A method of preparing a polypeptide containing one or more Aib residues using a 2-aminoisobutyric acid-tRNA synthetase (AibRS), wherein the AibRS comprises an amino acid sequence of SEQ ID NO:
- 7 or a variant thereof and having the function of aminoacylating tRNA with 2-aminoisobutyric acid (Aib), wherein the variant of SEQ ID NO:
- 7 retains 215Gly and is at least 90% identical to SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.