Ligase and dehydrogenase variants and methods of use

Abstract

The disclosure provides polypeptides and encoding nucleic acids of engineered acyl coenzyme-A ligases, engineered In acetylating aldehyde dehydrogenases or both. The disclosure also provides cells expressing an engineered form of the acyl coenzyme-A ligases, acetylating aldehyde dehydrogenases or both. The disclosure further provides methods for producing a bioderived compound, such as 6-hydroxyhexanoyl coenzyme-A, 6-hydroxy hexanal, 1,6 hexanediol, 6-aminocaproyl-CoA, 6-aminocaproate semialdehyde, hexamethylenediamine, and products derived therefrom comprising culturing cells expressing an engineered acyl coenzyme-A ligases, engineered acetylating aldehyde dehydrogenases or both.

Dependencies are inferred from the claim text.