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US 2026/0209729 A1Published application
Mutated Lipase
AssigneeWilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.
InventorsYaoji Xuan, Guorui Liu, Shengnan Wang, Xiaojun Dai
Life of this patent
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Abstract
The present invention relates to a mutated lipase. The present invention further relates to a gene encoding the lipase, and a vector and a host cell comprising the gene. In addition, the present invention further relates to use of the lipase.
Claims13
A lipase, comprising an amino acid sequence having a mutation relative to SEQ ID NO:
- 1 at one or more amino acid positions corresponding to positions 27, 38, 96, 111, 163, 254 and 256 of SEQ ID NO:
- 1;
- preferably, the mutation is one or more of D27R, G38A, D96E, D111A, G163K, D254S and A256T.
(canceled)
A method for preparing a lipase mutant, comprising introducing one or more mutations at amino acid positions of a starting lipase corresponding to positions 27, 38, 96, 111, 163, 254 and 256 of SEQ ID NO:
- 1, preferably, the mutation is one or more of D27R, G38A, D96E, D111A, G163K, D254S and A256T;
- optionally, the method further comprises introducing one or more mutations at amino acid positions of a starting lipase corresponding to positions 86, 93, 95 and 211 SEQ ID NO:
- 1, preferably, the mutation is one or more of L86I, 193L and M95F;
- L86I, 193L, M95F and L211F;
- L86I, 193L, M95Y and L211F;
- or L86I, 193L, M95W and L211F;
- preferably, the sequence of the starting lipase is as shown in SEQ ID NO:
- 1.
5 more elements
Dependencies are inferred from the claim text.