Phytase variants with improved properties
AssigneeBASF SE
InventorsArne Solbak, Stefan Haefner
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Abstract
The present invention relates to phytase variants with improved properties such as an increased thermostability and/or an increased gastric stability, nucleotide sequences encoding said phytases, processes for the preparation and the use of phytases and to animal feeds comprising these phytases.
Claims15
A polypeptide having phytase activity or fragment thereof having phytase activity, wherein the polypeptide or fragment thereof has an amino acid sequence which is at least 94% identical to the amino acid sequence as set forth in SEQ ID NO:
- 1 and wherein the polypeptide comprises at least one amino acid modification at an amino acid residue number selected from the group consisting of 71, 104, 192, 195, 201, 239, 243, 337, 360 and 365, compared to the amino acid sequence as set forth in SEQ ID NO:
- 1 and referring to the numbering of SEQ ID NO:
- 1.
A polypeptide having phytase activity or fragment thereof, wherein the polypeptide or fragment thereof has a melting temperature Tm of at least 101.5° C. as determined by differential scanning calorimetry using the conditions as described in Example 1.2.
A polypeptide having phytase activity or fragment thereof having phytase activity, wherein the polypeptide or fragment thereof has an amino acid sequence which is at least 95.9% identical to the amino acid sequence as set forth in SEQ ID NO:
- 2.
A method for producing a polypeptide, comprising culturing a host cell under suitable conditions, optionally further comprising recovering the polypeptide,
- wherein the host cell comprises a nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide having phytase activity, wherein the polypeptide has an amino acid sequence which is at least 94% identical to the amino acid sequence as set forth in SEQ ID NO:
- 1 and wherein the polypeptide comprises at least one amino acid modification at an amino acid residue number selected from the group consisting of 71, 104, 192, 195, 201, 239, 243, 337, 360 and 365, compared to the amino acid sequence as set forth in SEQ ID NO:
- 1 and referring to the numbering of SEQ ID NO:
- 1.
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Dependencies are inferred from the claim text.