Utr for improving translation efficiency and/or stability of rna molecule and use thereof
AssigneeShenzhen Shenxin Biotechnology Co., Ltd.
InventorsHui HUANG, Linxian LI
Life of this patent
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Abstract
Provided is a recombinant RNA molecule, which comprises a nucleotide sequence encoding a polypeptide of interest and 5′-UTR and/or 3′-UTR. The RNA molecule has improved translation efficiency and/or stability, and improves the expression level of a polypeptide and/or protein. Also provided are a vector encoding the recombinant RNA molecule, a pharmaceutical composition comprising the recombinant RNA molecule, and a method for treating or preventing a disease by using the recombinant RNA molecule.
Claims28
A recombinant RNA molecule, comprising:
- (1) a first nucleotide sequence encoding a polypeptide and/or protein of interest;
- and
- (2) a second nucleotide sequence comprising a 5′-untranslated region (5′-UTR);
- wherein the 5′-UTR comprises at least one polynucleotide selected from:
- (a) a 5′-UTR derived from at least one gene of genes PPIA, HPX, FTCD, CDK5RAP3, HSPA8, HBA1, MYSM1, LENG1, TMSB4X, CASP4, IFNA1, PGLYRP1, UCHL1, CPAMD8, TTR, APOA2, GH1, DTYMK, APOC2, and CDK7;
- (b) a fragment of the 5′-UTR in (a);
- (c) a variant of the 5′-UTR in (a);
- and
- (d) a variant of the fragment in (b);
- the first nucleotide sequence and the second nucleotide sequence are not naturally occurring in the same RNA molecule.
5-6. (canceled)
(canceled)
10-11. (canceled)
A recombinant RNA molecule, comprising:
- (1) a first nucleotide sequence encoding a polypeptide and/or protein of interest;
- and
- (2) a second nucleotide sequence comprising a 3′-untranslated region (3′-UTR);
- wherein the 3′-UTR comprises at least one polynucleotide selected from:
- (a) a 3′-UTR derived from at least one gene selected from genes MPND, FBXW10, FBXW12, PGLYRP1, HPX, CDK7, APOC2, PFN1, RBP4, FTCD, NAAA, GSDMD, FBXL8, ORM1, CASP4, CHMP2A, LENG1, MYCBPAP, APOC1, HSPA8, APOA2, UCHL1, TSG101, NAE1, and NFKB2;
- (b) a fragment of the 3′-UTR in (a);
- (c) a variant of the 3′-UTR in (a);
- and
- (d) a variant of the fragment in (b);
- wherein the first nucleotide sequence and the second nucleotide sequence are not naturally occurring in the same RNA molecule.
16-17. (canceled)
(canceled)
21-22. (canceled)
24-26. (canceled)
28-40. (canceled)
Dependencies are inferred from the claim text.