Species-Specific Tissue Culture Medium for the Propagation of Cells

Abstract

A cell culture medium is disclosed. The cell culture medium comprises: a species-specific cell culture medium comprising: bFGF of the species, PDGF-BB of the species, and EGF of the species; and serum of the species. Further disclosed is a canine cell culture medium comprising: canine bFGF, canine PDGF-BB, and canine EGF; and canine serum. A composition is described comprising: a medium comprising: basal medium, sodium bicarbonate, HEPES, L-glutamine, L-alanine, Insulin, hydrocortisone, progesterone, Asc-2-p, holo-transferrin; bFGF of the species, PDGF-BB of the species, and EGF of the species; and serum consistent with the cells of the species; MSC cells of the species. A method of producing exosomes is described, the method comprising: expanding mesenchymal stem cells and/or islet cells in culture, the culture and collecting exosomes released by the mesenchymal and/or adipose stem cells in culture.

Dependencies are inferred from the claim text.