Method for producing car-t cells
AssigneeTakeda Pharmaceutical Company Limited
InventorsSoichiro Ogaki, Shogo Saito, Eiki Maeda
Life of this patent
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Abstract
The present disclosure provides improved methods of producing engineered immune cells (e.g., CAR-T cells). The resulting engineered immune cells and compositions comprising the same are useful in treating various diseases, e.g., infection, autoimmune diseases, and tumors.
Claims25
A method of producing a population of engineered immune cells, the method comprising:
- (i) culturing a population of immune cells,
- (ii) contacting the population of immune cells with a nucleic acid molecule comprising a nucleotide sequence encoding a heterologous amino acid sequence, thereby providing the population of engineered immune cells, and
- (iii) harvesting the population of engineered immune cells,
- wherein step (i) and/or step (ii) is at least partly performed in the presence of (a) IL-15 and (b) IL-1b and/or IL-12.
A method of producing a population of engineered immune cells, the method comprising:
- (i) culturing a population of immune cells,
- (ii) contacting the population of immune cells with a nucleic acid molecule comprising a nucleotide sequence encoding a heterologous amino acid sequence, thereby providing the population of engineered immune cells,
- (iii) culturing the population of engineered immune cells derived from step (ii), and
- (iv) harvesting the population of engineered immune cells for storage or administration,
- wherein step (i), step (ii), and/or step (iii) is at least partly performed in the presence of (a) IL-15 and (b) IL-1b and/or IL-12.
A method of increasing a population of a subset of naive T cells or stem cell memory T cells comprising contacting a population of immune cells with (a) IL-15 and (b) IL-1b and/or IL-12.
A composition comprising a population of immune cells and (a) IL-15 and (b) IL-1b and/or IL-12.
A method of increasing a population of gamma delta T cells comprising contacting a population of gamma delta T cells with IL-12.
Dependencies are inferred from the claim text.