Culture methods

The method of any one of the preceding claims, wherein the aggregation is cell-mediated aggregation.

The method of any one of the preceding claims, wherein the culture period of (i) comprises all or part of the log phase of growth of the cell population.

The method of any one of the preceding claims, wherein the culture period is at least 24 hours.

The method of any one of the preceding claims, wherein the cell culture system is a continuous cell culture system or a batch cell culture system, optionally wherein the system comprises a bioreactor.

A cell culture vessel comprising a cell culture medium, two or more cell clusters and an exogenous protease, wherein the two or more cell clusters comprise a cell population in the log phase of growth and wherein the exogenous protease is an exogenous metalloprotease and/or cysteine protease.

The method or cell culture vessel of any one of the preceding claims, wherein the two or more cell clusters each comprise a microcarrier, and the microcarrier is a bead.

The method or cell culture vessel of any one of the preceding claims, wherein the two or more cell clusters each comprise a microcarrier and wherein the microcarrier comprises a material selected from the group consisting of:

• plastic, polymer, glass, and metal.

The method or cell culture vessel of any one of the preceding claims, wherein the cell population comprises cells selected from the group consisting of:

• skin, muscle, cervical, breast, and prostate cells.