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AssigneeDanisco US Inc.
InventorsLori Ann Maggio-Hall, Timothy Caspar, Carol M. McCutchen, Seung-Pyo Hong, Suzanne Koch Singles, George C. Fox, Sergio C. Nanita
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Certain one or more embodiments of the instant disclosure are related to, inter alia, recombinant (genetically modified) filamentous fungal cells (strains) producing proteins of interest, methods and compositions for the design and construction of modified filamentous fungal cells producing proteins of interest, methods and compositions for the expression/production/secretion/recovery and the like of endogenous and/or heterologous proteins of interest (e.g., phytases, lipases, glucoamylases, phospholipases, esterases, cellulases, hemicellulases, xylanases, etc.) in modified filamentous fungal cells, methods and compositions for producing proteins of interest in recombinant filamentous fungal, wherein the proteins produced and secreted into the broth and/or proteins recovered from the broth have uniform and consistent N-linked glycosylation patterns, and/or reduced (unwanted) glycation of one or more proteins of interest, and the like.
A modified filamentous fungal cell derived from a parental cell comprising an endogenous gene encoding a functional alpha-mannosidase protein and having an introduced expression cassette encoding a protein of interest (POI), wherein the modified cell comprises a genetic modification rendering the cell deficient in production of the functional alpha-mannosidase protein.
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A modified Trichoderma reesei cell derived from a parental T. reesei cell comprising a mutant glucosidase IIα (gls2aStop) allele encoding a truncated glucosidase II α-subunit (GIIαStop) protein, wherein the modified cell comprises a restored glucosidase IIα (gls2aR) allele encoding a native glucosidase II α-subunit (GIIα).
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A method for fermenting a filamentous fungal cell for the production and recovery of a protein of interest (POI) in a fermentation broth comprising a reduced amount of mannose (Man) sugars, the method comprising:
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The method of embodiment 4, wherein the parental cell is a Trichoderma sp. cell comprising a mutated glucosidase IIα (gls2aStop) allele encoding a truncated glucosidase II α-subunit (GIIαStop) protein, wherein the modified a T. reesei cell comprises a restored glucosidase IIα (gls2aR) allele encoding a native glucosidase II α-subunit (GIIα) protein.
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Dependencies are inferred from the claim text.