Cell culture container, cell culture device, and cell culture method
AssigneesHitachi, Ltd., Tokai University Educational System
InventorsTakayuki NOZAKI, Masaharu KIYAMA, Shizu TAKEDA, Yuki NIIMI, Kakuro HIRAI, Hiroko HANZAWA, Masato SATO, Miki KATO
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Abstract
Provided is a cell culture container that enables easy removal of a plurality of cell sheets cultured in one cell culture container. The cell culture container includes: a frame body that partitions the culture container; and a base member that is disposed on a bottom surface of the culture container for each of a plurality of sections partitioned by the frame body, in which the base member is removable for each of the sections.
Claims16
A cell culture container comprising:
- a frame body that partitions the culture container;
- and
- a base member that is disposed on a bottom surface of the culture container for each of a plurality of sections partitioned by the frame body,
- wherein the base member is removable for each of the sections.
A cell culture device comprising:
- a cell culture container that includes a frame body that partitions the culture container, and a base member that is disposed on a bottom surface of the culture container for each of a plurality of sections partitioned by the frame body, the base member being removable for each of the sections, the frame body having a height smaller than a height of a side wall of the culture container;
- a lid that covers an opening of the culture container;
- a liquid holding portion that stores a culture solution;
- a liquid feeding pipe that penetrates through the lid and supplies the culture solution from the liquid holding portion to the culture container;
- and
- a discharge pipe that penetrates through the lid and discharges the culture solution from the culture container.
A cell culture method comprising:
- supplying a culture solution containing cells to a culture container and culturing a cell sheet on a base member that is disposed in each of a plurality of sections partitioned by a frame body and is removable for each of the sections;
- removing the frame body;
- and
- acquiring the base member on which the cultured cell sheet is placed.
Dependencies are inferred from the claim text.