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US 2026/0209656 A1Published application
Genetically engineered yeast cells and methods of use thereof
AssigneeBerkeley Fermentation Science Inc.
InventorsJeremy ROOP, Charles DENBY, Rachel LI, Nicholas HARRIS
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Abstract
Provided herein are genetically modified yeast cells that recombinantly expresses a gene encoding a mutant beta-lyase. Also provided are methods of producing fermented products and methods of producing ethanol.
Claims23
A genetically modified yeast cell, comprising a heterologous gene encoding an enzyme with beta-lyase activity, wherein when used for fermenting unhopped wort, the genetically modified yeast cell:
- a. produces less than 150 μg/L indole and at least 200 ng/L 3-mercaptohexan-1-ol (3-MH);
- or
- b. produces less than a 1.5-fold increased concentration of indole and at least a 5-fold increased concentration of 3-MH, as compared to a cell from which the genetically modified yeast cell is derived.
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Dependencies are inferred from the claim text.